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Table 1 Sampling information on the green crab Carcinus aestuarii including collection sites, countries, Mediterranean basins, regions, geographic coordinates, and number of examined specimens (N) per each location. Genetic diversity measures (including number of haplotype (Nh), number of polymorphic sites (Nps), haplotype (h) and nucleotide (π) diversities, and mean number of nucleotide differences (K)) and historical demographic results (inferred from: Tajima’s D test (D), Fu’s F S test (F S ), Ramos-Onsins and Rozas’s R2 test (R2), and mismatch distribution raggedness index (rg)) were also provided for each investigated location and the total dataset

From: Parapatric genetic divergence among deep evolutionary lineages in the Mediterranean green crab, Carcinus aestuarii (Brachyura, Portunoidea, Carcinidae), accounts for a sharp phylogeographic break in the Eastern Mediterranean

Collection site Country Mediterranean Basin Region Geographic coordinates N Nh Nps h π K D F S R 2 rg
Tabarkab Tunisia Western Mediterranean Algerian Basin 36°57′N 08°45′E 10 5 6 0.756 ± 0.130 0.0023 ± 0.0006 1.355 −1.492 −1.507 0.137 0.042
Bizerteb Tunisia Western Mediterranean Algerian Basin 37°16′N 09°52′E 15 8 10 0.867 ± 0.067 0.0030 ± 0.0005 1.771 −1.613 −3.541 0.083 0.064
Sidi Raisb Tunisia Western Mediterranean Algerian Basin 36°46′N 10°32′E 11 5 21 0.709 ± 0.137 0.0072 ± 0.0045 4.236 −1.859 1.435 0.258 0.094
Kelibiab Tunisia Western Mediterranean Afro-Sicilian Basin 36°51′N 11°05′E 10 5 6 0.667 ± 0.163 0.0023 ± 0.0008 1.355 −1.492 −1.507 0.137 0.037
Benikhiarb Tunisia Western Mediterranean Afro-Sicilian Basin 36°28′N 10°46′E 12 2 1 0.167 ± 0.134 0.0002 ± 0.0002 0.166 −1.140 −0.475 0.276 0.472
Monastirb Tunisia Eastern Mediterranean Afro-Sicilian Basin 36°10′N 10°49′E 11 9 28 0.945 ± 0.066 0.0138 ± 0.0051 8.127 −0.692 −1.391 0.120 0.054
Chebbab Tunisia Eastern Mediterranean Afro-Sicilian Basin 35°14′N 11°07′E 12 11 31 0.985 ± 0.040 0.0160 ± 0.0039 9.424 −0.369 −3.052 0.118 0.051
Sfaxb Tunisia Eastern Mediterranean Afro-Sicilian Basin 34°44′N 10°45′E 15 12 27 0.943 ± 0.054 0.0166 ± 0.0025 9.752 0.729 −2.077 0.160 0.026
Djerbab Tunisia Eastern Mediterranean Afro-Sicilian Basin 33°52′N 10°51′E 14 9 29 0.912 ± 0.059 0.0186 ± 0.0022 10.967 0.867 0.813 0.166 0.044
Tripolib Libya Eastern Mediterranean Afro-Sicilian Basin 32°54′N 13°11′E 11 6 23 0.891 ± 0.063 0.0089 ± 0.0043 5.254 −1.512 0.848 0.227 0.095
Mosratab Libya Eastern Mediterranean Afro-Sicilian Basin 32°22′N 15°05′E 12 8 28 0.894 ± 0.078 0.0150 ± 0.0044 8.833 − 0.212 0.420 0.140 0.094
Venice Lagoonb Italy Eastern Mediterranean Adriatic Sea 45°27′N 12°16′E 11 7 10 0.909 ± 0.066 0.0060 ± 0.0009 3.563 0.186 −1.205 0.149 0.064
Pomera Croatia Eastern Mediterranean Adriatic Sea 44°49′N 13°53′E 20 15 19 0.953 ± 0.035 0.0047 ± 0.0008 2.810 1.797 −11.055 0.052 0.046
Amvrakikosa Greece Eastern Mediterranean Ionian Sea 39°1.2’N20°45.3′E 13 7 11 0.731 ± 0.133 0.0028 ± 0.0008 1.692 −2.112 −2.833 0.093 0.044
Lefkada Greece Eastern Mediterranean Ionian Sea 38°49.6’N 20°43′E 14 8 27 0.824 ± 0.098 0.0088 ± 0.0045 5.208 −1.648 −0.315 0.184 0.042
Navarinoa Greece Eastern Mediterranean Ionian Sea 36°57’N 21°39.7′E 10 7 26 0.867 ± 0.107 0.0220 ± 0.0040 12.911 1.930 1.481 0.230 0.241
Alexandroupolis Greece Eastern Mediterranean Aegean Sea 40°51′N 25°52′E 12 7 20 0.773 ± 0.128 0.0083 ± 0.0024 4.893 −1.146 − 0.074 0.097 0.053
Izmir Bay Turkey Eastern Mediterranean Aegean Sea 38°27′N 27°05′E 6 3 4 0.600 ± 0.215 0.0022 ± 0.0009 1.333 −1.295 0.296 0.235 0.737
Enez Dalyan Lagoon Turkey Eastern Mediterranean Aegean Sea 40°42′N 26°03′E 20 13 23 0.853 ± 0.080 0.0050 ± 0.0014 2.973 −2.081 −6.804 0.062 0.055
Dardanelles Strait Turkey Eastern Mediterranean Sea of Marmara 40°13′N 26°32′E 5 3 12 0.700 ± 0.218 0.0081 ± 0.0036 4.800 −1.205 2.225 0.291 0.470
Prince’s Islands Turkey Eastern Mediterranean Sea of Marmara 40°52′N 29°05′E 12 6 16 0.758 ± 0.122 0.0065 ± 0.0022 3.833 −1.193 0.323 0.142 0.084
Bosphorus Strait Turkey Eastern Mediterranean Sea of Marmara 41°12′N 29°06′E 7 6 7 0.952 ± 0.096 0.0050 ± 0.0007 2.952 0.172 −2.275 0.165 0.065
Total      263 106 97 0.912 ± 0.012 0.0211 ± 0.0009 12.389 − 0.650 −23.885 0.062 0.023
  1. Significant values are in bold. Non-significant values for the raggedness index (rg) accept the null hypothesis of expectation under a sudden demographic expansion model. a: Data examined by Ragionieri and Schubart [35]. b: Data examined by Deli et al. [12]. Examined specimens of the population of Navarino (labelled as Peloponnesus in Ragionieri and Schubart [35]) were those assigned to Cox1 type I