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Fig. 6 | BMC Evolutionary Biology

Fig. 6

From: Comparison of ventral organ development across Pycnogonida (Arthropoda, Chelicerata) provides evidence for a plesiomorphic mode of late neurogenesis in sea spiders and myriapods

Fig. 6

Cell proliferation in the VNC of postlarval and juvenile T. orbiculare instars. Labeling of acetylated tubulin (white) and EdU (red) with nuclear counterstain (blue) after 4 h EdU exposure. Maximum projections (a, c) and optical sections (b, b’, d, d’) of wholemount CNSs, anterior to the top. Stippled ovals highlight the internal VOs of one body half. Stars indicate the central cavity around which tubulin-rich cell processes converge. White arrows indicate broad tubulin-rich cell bands and condensed fibrous strands extending dorsally from the VOs towards the neuropil. White arrowheads mark single EdU-positive cells in the periphery of the ganglia. Asterisks highlight transient posterior ganglion anlagen. a: CNS of last postlarval instar, ventral view, EdU labeling shown separately on the right. Note high cell proliferation in palpal and ovigeral VOs as compared to the posteriorly adjacent VOs. b & b’: Wlg2–4, horizontal and sagittal sections. Black arrows highlight selected larger nuclei in the VOs of wlg4. Black arrowheads mark mitotic cells with asymmetrically positioned metaphase plates. c: CNS of the first juvenile instar, ventral view, EdU labeling shown separately on the right. d & d’: Wlg2–4, horizontal and sagittal sections. Only few large VO nuclei remain, VO nuclei being slightly smaller than the neuronal nuclei in the soma cortex. Abbreviations: br – brain, ovn – ovigeral neuromere, pan – palpal neuromere, seg – subesophageal ganglion, wlg – walking leg ganglion, wln – walking leg neuromere

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