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Fig. 10 | BMC Evolutionary Biology

Fig. 10

From: Comparison of ventral organ development across Pycnogonida (Arthropoda, Chelicerata) provides evidence for a plesiomorphic mode of late neurogenesis in sea spiders and myriapods

Fig. 10

Asymmetric divisions and cell cycle aspects of VO cells of C. brevirostris and T. orbiculare. Horizontal optical sections through walking leg ganglion 4 of the last postlarval instars of C. brevirostris (a, b) and T. orbiculare (c, d). EdU labeling (4 h exposure, red) coupled to acetylated tubulin (white) and nuclear counterstain (blue). PH3 labeling (a, b) shown in yellow. Stippled ovals highlight the internal VOs of one body half. Stars indicate the central cavity around which tubulin-rich cell processes converge. a, b Advanced mitotic stages (=metaphase and later) of the VO cells of C. brevirostris show no EdU co-labeling, whereas earlier mitotic phases have weak EdU signal (black arrowheads). Note the asymmetry in the division of one large neural precursor cell (stippled outline). c, d Mitotic VO cells in metaphase and even telophase are EdU-positive and show indications of morphologically asymmetric divisions (stippled outlines). The black arrowhead marks a potential pycnotic body indicative of cell death occurring in addition to cell proliferation

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