Table 3 Gene-specific oligonucleotides for expression analyses and cDNA cloning of horse CEA gene family members
From: Alternative splicing after gene duplication drives CEACAM1-paralog diversification in the horse
Gene | Oligonucleotide sequence | Location of primers (exon) | Size of PCR product (bp) |
|---|---|---|---|
CEACAM1 | For: TGCATCATATAAGATAGGCCCAG | N domain | 367 |
Rev: AGTGAGAGTCCTCTTGTCCAGG | A1 domain | ||
CEACAM41 | For: CATTGCATGTGATAGAGCGAC | N domain | 246 |
Rev: CGTCCTTCTGTTCTGTGACTGT | A2 domain | ||
CEACAM42 | For: AGGGGAAGGAATAGATCCCG | N domain | 392 |
Rev: GAGTCCTGTTGTCCGAGGATAG | A1 domain | ||
CEACAM43 | For: CCCATCAAGAAATTGTGTCCT | N domain | 276 |
Rev: ATGTTAACACTACAGGGTCCCTG | A1 domain | ||
CEACAM44 | For: GCTGTTGTAGGGACCGATGTTA | N domain | 503 |
Rev: CCTCCTTCCTGATGATGTGTGT | A2 domain | ||
CEACAM45 | For: GCGATAGGGCAACAAGAAATTAT | N domain | 254 |
Rev: CATGTTAACACTACAGGGTCCTCA | A1 domain | ||
CEACAM46 | For: AGTCCCACCCAATGGTATCC | N domain | 525 |
Rev: CCCAAGTATTGCCCCTTCTGT | A1 domain | ||
CEACAM47 | For: ACAGACCAAGTCCCAAAACC | N domain | 256 |
Rev: TTACGTCTTTTGACGATTCCAG | A1 domain | ||
CEACAM48 | For: GACCAGCTCGCAAACAAA | N domain | 279 |
Rev: AACAAGCTTCTTATCTGGCATTT | A2 domain | ||
CEACAM49 | For: TGGAGCACGTCCACATAAAC | N domain | 232 |
Rev: GGAGGTATTTGACCCTGGATT | A2 domain | ||
CEACAM50 | For: AGATGCTCTTGAAGGAACGGAT | N domain | 453 |
Rev: GACAGCTTCAGCCAGGTCCTA | A1 domain | ||
CEACAM51 | ND | NA | NA |
CEACAM52 | For: ATGCTGCTGCAGGGGATA | N domain | 508 |
Rev: CATCCTCCCTCCTGACACAT | A2 domain | ||
CEACAM53 | For: TTCAAAGGGGAAATAGATTCCA | N domain | 394 |
Rev: GAGTCCTGTTGTCTGGGGAC | A1 domain | ||
CEACAM54 | For: ATCAGTCCCTGGCTTCAGA | N domain | 409 |
Rev: TACACGGAGCTGTATACTTC | A domain | ||
CEACAM55 | For: CCCTACTAGTCACGAGGAAGAAC | N domain | 222 |
Rev: CATCCTCTCGGTCAGTCACA | A2 domain | ||
CEACAM1 | For: GTCAGTAAGCTTCAGCAGACACCATGGAACTC | 5’UTR | 1624 |
Rev: GTCAGTTCTAGACAGTGAACAGGGCAGGATATG | 3’UTR | ||
CEACAM41 | For: GAGCAGTGCTTGTGAGCATT | 5’UTR | 1131 |
Rev: TAAGGGGAAGTTCCTGAAGG | 3’UTR | ||
CEACAM42 | For: TCACAGAGGGAGGGACAGAG | 5’UTR | |
Rev: GGGTAAAGGGATCCTTCCAG | 3’UTR | 1390 | |
Rev: TCACAGAGGGAGGGACAGAG | TM domain | 1121 | |
CEACAM43 | For: GTCAGTAAGCTTGACAGAGCAGGCAGCAGAC | 5’UTR | 1613 |
Rev: GTCAGTTCTAGAGAACAGGGCAGGTTGCAT | 3’UTR | ||
CEACAM45 | For: TCACAGAGGAAAGGACAGAGC | 5’UTR | |
Rev: CCTTGATTCCTGGACATTGAA | 3’UTR | 1539 | |
Rev: CCTTGATTCCTGGACATTGAA | TM domain | 1379 | |
CEACAM50 | For: CAGGAGTGCTTGTGAGAGTT | 5’UTR | 1139 |
Rev: AGGGAAGAGGCTTCGTCTTC | 3’UTR | ||
CEACAM52 | For: GTCAGTAAGCTTGCAGAAGCTCATCTCACAGAG | 5’UTR | 583 |
Rev: GTCAGTTCTAGATTGTGAGGCAGATCAGATCC | 3’UTR | ||
CEACAM53 | For: CAGAGGGAGGGACAGAACAG | 5’UTR | 1163 |
Rev: ATGGCAGTTAGCCTTGGAGA | 3’UTR | ||
CEACAM54 | For: TCTCACAGAGGGAGGAGCCA | 5’UTR | 916 |
Rev: AGTCAGCAGTGCAGGAAACA | 3’UTR |