Fig. 6

CEACAM41 is a secreted protein. a PBMC were cultured with 500 U/ml rhIL-2 for the indicated times. RT-PCR using primers located in the leader and the N-domain suggested that CEACAM41 is preferentially expressed by stimulated PBMC. Full length amplification of CEACAM41 cDNA identified two different transcripts (b). The two transcripts differ from each other by a short sequence, inserted between exon C2 and C3 further named C2’ exon (c). The stop codon which exists in the extended C1 exon is indicated by the red arrow and the “stop” (c). Both transcripts code for the same protein (d). e Comparison of the amino acid sequence of the cytoplasmic tail of CEACAM41 as predicted from the exon sequence of the CEACAM41 gene and the peptide sequence encoded by the cytoplasmic exons in transcripts without transmembrane domains