Fig. 3

CEACAM42 has an extended transmembrane domain. The exon structure of the CEACAM42 gene indicates that its gene product may contain an ITIM signaling motif. However as shown in a the cloned mRNA demonstrate that an alternative splice donor site of the transmembrane exon is used and that the exons C1 was not integrated into the mRNA. The stop codon which exists in the extended transmembrane exon is indicated by the red arrow and the “stop”. The mutated splice donor sites of C1 is indicated with a red tilde. b shows the possible but unused splice donor site (underlined). c Amino acid sequence of the transmembrane and cytoplasmic part of CEACAM42. The PKA phosphorylation site is highlighted in blue, the predicted Y-based sorting signal in red. d Expression of CEACAM42 was detected in various parts of the intestine