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Fig. 4 | BMC Evolutionary Biology

Fig. 4

From: The function and evolutionary significance of a triplicated Na,K-ATPase gene in a toxin-specialized insect

Fig. 4

a survivorship assay for α1A, α1B and α1C knockdown bugs 5 days post administration of dsRNA. b Survivorship assay for bugs post injection with the cardenolides ouabain and digoxin. Neg = negative control, not injected; Pos = positive control, injected with eGFP dsRNA. Error bars represent standard errors of mean values. c Efficacy of the sequence-verified and copy-specific RNAi knockdowns for the gene copies α1A, α1B and α1C. Normalized fold change is relative to the eGFP-injected control in each case. Error bars represent standard errors of mean values

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