Fig. 5

Immunofluorescence images of DRP1 localization in Gonium pectorale. Differential interference contrast (DIC) images (a, e, i, m), immunofluorescence images labeled with an anti-TsDRP1 (b, f, j, n) and an anti-tubulin alpha (c, g, k, o), and merged immunofluorescence images of DRP1 and tubulin (d, h, l, p) are shown. In vegetative cells, DRP1 was localized to cytoplasm (a-d). DRP1 was localized to a first division plane of two-celled embryo (white arrowheads in f-h), both first and second division planes of four-celled embryo (j-l), all first, second, third division planes of eight-celled embryo (n-p). The second division plane in four-celled stage was estimated by strong immunofluorescence of the anti-tubulin alpha (phycoplast). Scale bars: 10 μm