Fig. 4From: Evolution of cytokinesis-related protein localization during the emergence of multicellularity in volvocine green algaeImmunofluorescence images of DRP1 localization in Tetrabaena socialis. Differential interference contrast (DIC) images (a, e, i), immunofluorescence images labeled with an anti-TsDRP1 (b, f, j) and an anti-tubulin alpha (c, g, k), and merged immunofluorescence images of DRP1 and tubulin (d, h, l) are shown. In vegetative cells, DRP1 was localized to cytoplasm (b-d). DRP1 was localized to a first division plane (white arrowheads in f-h) of two-celled embryo and both first (white arrowheads in j-l) and second (blank arrowheads in j-l) division planes of four-celled embryo. The first and second division planes in the four-celled stage were estimated by directions of the planes within the parental colony. Scale bars: 10 μmBack to article page