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Fig. 4 | BMC Evolutionary Biology

Fig. 4

From: Evolution of cytokinesis-related protein localization during the emergence of multicellularity in volvocine green algae

Fig. 4

Immunofluorescence images of DRP1 localization in Tetrabaena socialis. Differential interference contrast (DIC) images (a, e, i), immunofluorescence images labeled with an anti-TsDRP1 (b, f, j) and an anti-tubulin alpha (c, g, k), and merged immunofluorescence images of DRP1 and tubulin (d, h, l) are shown. In vegetative cells, DRP1 was localized to cytoplasm (b-d). DRP1 was localized to a first division plane (white arrowheads in f-h) of two-celled embryo and both first (white arrowheads in j-l) and second (blank arrowheads in j-l) division planes of four-celled embryo. The first and second division planes in the four-celled stage were estimated by directions of the planes within the parental colony. Scale bars: 10 μm

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