Fig. 3

Immunofluorescence images of DRP1 localization in Chlamydomonas reinhardtii. Differential interference contrast (DIC) images (a, e, i), immunofluorescence images labeled with an anti-TsDRP1 (b, f, j) and an anti-tubulin alpha (c, g, k), and merged immunofluorescence images of DRP1 and tubulin (d, h, l) are shown. In vegetative cells, DRP1 was localized to cytoplasm (b-d). DRP1 was localized to a first division plane of two-celled embryo (white arrowheads in f-h) and second division planes of four-celled embryo (black arrowheads in j-l) mainly. Note that DRP1 signals localized to the first division plane of the two-celled embryo (f) become weak in the four-celled stage (j). The first and second division planes in the four-celled stage were estimated by relative positions of pyrenoids. Scale bars: 5 μm