Fig. 4

Intrageneric and intergeneric polymorphism of chloroplast variation regions of three Primulina species and Boea hygrometrica. Intrageneric (a) and intergeneric (b) polymorphism (PICs) of noncoding regions of cp genomes. Intrageneric (c) and intergeneric (d) polymorphism (PICs) of Con_Sea regions of cp genomes. PICs were counted by the sum of SNP and Indel between two cp genomes. Con_Sea is a region between two adjacent Con_Islands, which is defined as the regions containing over 50 continuous conserved sites in cross-genus consensus genome sequences. Eight regions used for experimental evaluation were signed as filled circles, while others were empty circles. The name of the regions with high variation or used for experimental evaluation was marked along the circles. It was noting that the name of these regions in part C and D was shown as the name of noncoding regions overlapped with corresponding Con_Seas, instead of original ID of Con_Seas. The yellow lines link the same regions, while the red lines link the corresponding regions between noncoding regions and Con_Seas