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Fig. 6 | BMC Evolutionary Biology

Fig. 6

From: Mutual maintenance of di- and triploid Pelophylax esculentus hybrids in R-E systems: results from artificial crossings experiments

Fig. 6

Detection of micronuclei in gonads of hybrid tadpoles. Morphological analysis of gonads dissected from P. ridibundus (a, b) tadpoles, diploid (e, g, h) and triploid (c, d, f, i, j) P. esculentus tadpoles. Undifferentiated gonads (stage 3) from P. ridibundus tadpoles (a). Gonads at stage of high mitotic activity (stage 4) from P. ridibundus tadpoles (b). Scale bars = 50 μm. Micronuclei (arrows) were abundant in cytoplasm of germ cells in gonads from diploid (e, g, h) and triploid (c, d, f, i, j) hybrid tadpoles. Micronuclei vary in size and chromatin density. Anaphase stage of mitosis (h, i, j); no lagging chromosomes (arrowheads) was observed. Scale bar for c = 50 μm; d = 25 μm; e-j = 10 μm

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