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Fig. 3 | BMC Evolutionary Biology

Fig. 3

From: Mutual maintenance of di- and triploid Pelophylax esculentus hybrids in R-E systems: results from artificial crossings experiments

Fig. 3

FISH mapping of interstitial (TTAGGG)n repeat sites allows to identify karyotypes of tadpoles resulting from artificial crosses of hybrid animals. Metaphase chromosomes of tadpoles after FISH with (TTAGGG)5. One or two interstitial (TTAGGG)n repeat sites (indicated by arrows) are distinguished in parental NOR-bearing chromosomes. Karyotypes of tadpoles resulting from crosses of triploid hybrid female with RRL genotype and diploid hybrid male (a, b), triploid hybrid female with LLR genotype and triploid hybrid male with RRL genotype (c, d) and two diploid hybrid frogs (e, f, g, h). Tadpoles were identified as P. ridibundus (b, c, g), diploid hybrids (a, d, h) and triploid hybrids with RRL (e) and LLR (f) genotypes. Scale bars = 10 μm

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