Fig. 4
From: Conservation and diversification of small RNA pathways within flatworms
Conserved regions in M. lignano GW182 family member. GW182 interacts with Ago and ‘label’ mRNAs for decay. A GW182 family member has two protein domains; a central Ubiquitin domain (UBA), and a RNA recognition motif (RRM), and unstructured regions in between domains. The N-termini region (N-GW) binds to Ago, and the middle (M-GW) and carboxi-terminal region (C-GW) mediate the decay. Each of these regions consists on a variable number of GW repeats (number between brackets). Additionally, there is a low complex region rich in glutamine (Q-rich) between UBA domain and M-GW region. There are two highly conserved sequences in N-GW (yellow box) and M-GW (green box) regions that we found to be conserved in M. lignano sequence