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Table 2 Estimation of mutation supply during experimental evolution

From: The path to re-evolve cooperation is constrained in Pseudomonas aeruginosa

Iron availability

Population bottleneck [CFU]

Number of cell divisionsa

Expected number of mutation eventsb

at specific nucleotide in pvdS

anywhere in pvdS locusc

Low

5.0 × 104

1.2 × 1010

12.3

8.2 × 104

Medium

1.3 × 105

3.1 × 1010

30.9

20.6 × 104

High

2.8 × 105

6.8 × 1010

67.8

45.1 × 104

  1. aacross all replicate populations and 20 transfers
  2. bassuming a mutation rate of ~10-9 per nucleotide per cell division for P. aeruginosa PAO1 [32]
  3. c pvdS gene and upstream promoter region consisting of 666 bp