Skip to main content
Fig. 5 | BMC Evolutionary Biology

Fig. 5

From: Morphogenesis along the animal-vegetal axis: fates of primary quartet micromere daughters in the gastropod Crepidula fornicata

Fig. 5

Anti-acetylated tubulin staining (red fluorescence) revealing ciliated cells in developing embryos. Embryos are oriented with the future posterior pole toward the bottom of the figure. Nuclei are labeled with DAPI (blue fluorescence). Specific cells/clones are as labeled. a, b Animal and vegetal views during early gastrulation, respectively. No ciliation is present at this stage. c, d Animal and vegetal views, respectively, during intermediate stages of gastrulation when ciliation is present in some 1q2 daughter cells, as labeled. e, f Animal-dorsal, and vegetal views later during gastrulation, respectively. Note group of four large ciliated cells on the dorsal surface in addition to 1a2 and 1b2. g, h Dorsal and ventral views, respectively, at an even later stage of gastrulation. Note even closer location of 1a2 and 1b2 relative to the four large ciliated cells on the dorsal surface (g). I-L Dorsal, right-lateral, ventral and left-lateral views of elongating embryo, respectively. Ciliated cells of the neurotroch (3c2 and 3d2 progeny) are seen within and posterior to the stomodeum [29]. The ciliated 1q2 cells along with 1d121 and 1d122 now occupy a large area on the dorsal-lateral surfaces of the future head. m-p Dorsal, right-lateral, ventral and left-lateral views, respectively, of a later stage when the embryo is undergoing elongation. Note large area occupied by ciliated cells on the dorsal surface of the head that contribute to the “provisional epithelium.” Additional cells have developed ciliation including progeny of 1b1, which will contribute to the anterior head field and prototroch, in addition to some progeny of 2b1 and 2b2. q-t Later stage of development when organ rudiments form. A large area is occupied by the provisional epithelium on the dorsal surface of the head. u-x Very early veliger larval stage. At this stage the ciliated prototroch cells have formed. There are ciliated cells in the head field and along the ventral midline in the foot (derived from 3c2 and 3d2). bp, blastopore; fr, foot rudiment; ft., foot; st, stomodeum; tc, terminal (“anal”) cells; vl, velar lobes; vr, velar rudiment. Scale bar in X equals 50 μm

Back to article page