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Fig. 4 | BMC Evolutionary Biology

Fig. 4

From: Morphogenesis along the animal-vegetal axis: fates of primary quartet micromere daughters in the gastropod Crepidula fornicata

Fig. 4

Live images showing examples of specific clones at various stages of development, as labeled. Individual cells were initially injected at the 16- to 28-cell stages. a Three examples of freshly injected 1q2 cells prior to their division (i.e., 1a2 and 1d2 at 28-cell stage, as labeled). Note the small apparent size of these micromere daughters. Nuclei were pre-labeled by expression of green GFP-histone H2B. Red fluorescent diI has been pressure microinjected into single cells using a fine glass needle, as shown. b-f 1a1 and 1a2 progeny, as labeled. b, c Dorsal-animal views of elongating embryos. d, e Left-lateral views of pre-veliger larvae undergoing organogenesis. f Anterior-dorsal view of an early veliger larvae. g-l 1b1 and 1b2 progeny, as labeled. g Animal view with the d quadrant located towards the bottom of the figure. h Dorsal-animal view. i Ventral view. j, k Right lateral views of pre-veliger larvae undergoing organogenesis. l Right lateral view of veliger larva. m-r 1c1 and 1c2 progeny, as labeled. m Animal pole view. n Dorsal-animal view. o Dorsal view. p-q Right-lateral views of pre-veliger larvae undergoing organogenesis. r Anterior-dorsal view of an early veliger larvae. s-x 1d1 and 1d2 progeny, as labeled. s-t Dorsal views of elongating embryos. u-v Right- and left-lateral views, respectively, of pre-veliger larvae undergoing organogenesis. w-x Anterior-dorsal and right-lateral views of early veliger larvae, respectively. To facilitate the injections embryos were pre-labeled by expression of either green GFP-histone H2B (as shown in a, g, h, n, q) or GFP-UTPH (as shown in b, c, i, m, s, t, v) protein. Unless noted otherwise, embryos are oriented with the posterior pole directed toward the bottom of the figure. Scale bar in X equals 50 μm

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