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Fig. 5 | BMC Evolutionary Biology

Fig. 5

From: Genetic basis of brain size evolution in cetaceans: insights from adaptive evolution of seven primary microcephaly (MCPH) genes

Fig. 5

In vitro assay for protein-protein interaction between ASPM IQ motif and calmodulin (CaM). a GST pull-down assay indicating a possible interaction of the ASPM IQ motif and CaM. Latin name of species shorthand initial capital letter for the genus and the first three letters of species listed upper gel-image. b Quantitative analysis of interaction of ASPM IQ and CaM using BLI analysis. The association and dissociation of increasing concentrations of ASPM IQ motif to CaM were shown. c Comparison of dissociation constants (Kd) values derived from BLI analysis. P values were calculated by Student’s t-test

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