Table 1 List of M. xanthus strains and plasmids used in this study
From: Divergence of functional effects among bacterial sRNA paralogs
Strain | Genotype | pxr allele description | Reference/source |
|---|---|---|---|
GJV1 | Derivative isolate of Myxococcus xanthus DK1622 | M. xanthus GJV1 pxr | |
GVB207.3 | Evolutionary descendant of GJV1 (herein “OC”) | M. xanthus GJV1 pxr | |
GJV207 | GVB207.3 Δpxr (herein “OC Δpxr”) | In-frame deletion of pxr | [7] |
NY01 | GJV207 intergenic::pPxrGJV1, kanR | M. xanthus GJV1 pxr | This study |
NY02 | GJV207 intergenic::pPxrnull, kanR | Intergenic region preceding pxr only | This study |
KC00 | GJV207 intergenic::pPxrAnc, kanR | The inferred pxr ancestor shared by Myxococcus and Cystobacter spp. | This study |
KC01 | GJV207 intergenic::pPxrMxs33, kanR | Myxococcus stipitatus Mxs 33 pxr | This study |
KC02 | GJV207 intergenic::pPxrMxs42, kanR | Myxococcus stipitatus Mxs 42 pxr | This study |
KC03 | GJV207 intergenic::pPxr Cb.1 , kanR | pxr Cb.1 in both Cystobacter minor Cbm 6 and Cystobacter violaceus Cbvi 34 | This study |
KC04 | GJV207 intergenic::pPxr Cbm6.2 , kanR | pxr Cbm6.2 in C. minor Cbm 6 | This study |
KC05 | GJV207 intergenic::pPxr Cbvi34.2 , kanR | pxr Cbvi34.2 in C. violaceus Cbvi 34 | This study |
KC06 | GJV207 intergenic::pPxr Cb.3 , kanR | pxr Cb.3 in both C. minor Cbm 6 and C. violaceus Cbvi 34 | This study |
KC07 | GJV207 intergenic::pPxr Sga , kanR | Stigmatella aurantiaca DSM 17044 pxr | This study |
Plasmid | Description | Reference/source | |
pCR2.1 | Cloning vector | Invitrogen | |
pPxrGJV1 | pCR2.1 with a 537-nt fragment that contains the 429-nt intergenic fragment and the 108-nt pxr coding region in M. xanthus | This study | |
pPxrnull | pCR2.1 with only the 429-nt intergenic fragment in M. xanthus | This study | |
pPxrAnc | pCR2.1 with the 429-nt intergenic fragment in conjunction with the inferred pxr ancestor shared by Myxococcus and Cystobacter. spp. | This study | |
pPxrMxs33 | pCR2.1 with the 429-nt intergenic fragment in conjunction with the pxr allele from M. stipitatus strain Mxs 33 | This study | |
pPxrMxs42 | pCR2.1 with the 429-nt intergenic fragment in conjunction with the pxr allele from M. stipitatus strain Mxs 42 | This study | |
pPxrCb.1 | pCR2.1 with the 429-nt intergenic fragment in conjunction with pxr Cb.1 in both C. minor Cbm 6 and C. violaceus Cbvi 34 | This study | |
pPxrCbm6.2 | pCR2.1 with the 429-nt intergenic fragment in conjunction with pxr Cbm6.2 in C. minor strain Cbm 6 | This study | |
pPxrCbvi34.2 | pCR2.1 with the 429-nt intergenic fragment in conjunction with pxr Cbvi34.2 in C. violaceus strain Cbvi 34 | This study | |
pPxrCb.3 | pCR2.1 with the 429-nt intergenic fragment in conjunction with pxr Cb.3 in both C. minor Cbm 6 and C. violaceus Cbvi 34 | This study | |
pPxrSga | pCR2.1 with the 429-nt intergenic fragment in conjunction with the pxr allele from S. aurantiaca strain DSM 17044 | This study | |