Fig. 3From: Contrasting morphology with molecular data: an approach to revision of species complexes based on the example of European Phoxinus (Cyprinidae)Haplotype networks constructed with nuclear DNA. The colours represent lineages detected by mitochondrial DNA analysis. For both genes, the gametic phase of heterozygous individuals was determined using Phase 2.1 [38, 39], then an unrooted minimum-spanning networks were constructed with median-joining algorithm [32] implemented in Network 5.1 (www.fluxus-engineering.com) with default settings. Figure 3a Rhodopsin haplotype network was constructed from 782 base pair long phased alignment. Figure 3b Recombination activating gene 1 (RAG1) haplotype network was constructed using 1413 base pair long phased alignment. The arrows denote areas, where hybrids were detected and correspond to Fig. 1 Back to article page