Fig. 2From: Contrasting morphology with molecular data: an approach to revision of species complexes based on the example of European Phoxinus (Cyprinidae)Phylogenetic reconstruction and haplotype network for revision of the genus Phoxinus. Figure 2a Phylogenetic tree constructed from the barcoding region of mitochondrial gene cytochrome oxidase I (COI). Collapsed alignment includes 139 unique haplotypes. The tree was created using Bayesian inference (BI) with BEAST 1.8.0 [26]. Branches carry posterior probabilities (PP) and bootstraps (BS) from the tree constructed with the Maximum-Likelihood (ML) method (PhyML; [29]). Weakly supported nodes are grey and only PP above 0.9 are shown for the main clades (no subclades). -, denotes lack of bootstraps originating from the difference between the BEAST and ML trees. Figure 2b Phylogenetic tree constructed with two partitions: COI and cytochrome b. Collapsed alignment includes 162 unique haplotypes. As in Fig. 2a , BI and ML were used. Because PhyML does not support partitions, GARLI v.2.01 [30, 31] was used for ML. Figure 2c An unrooted minimum-spanning network was constructed with COI using the median joining algorithm [32] implemented in Network 5.0 (www.fluxus-engineering.com) with default settings. The position of the type material in the network is denoted with TYPE PM for Phoxinus marsilii and TYPE PC for P. csikii Back to article page