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Fig. 2 | BMC Evolutionary Biology

Fig. 2

From: Contrasting morphology with molecular data: an approach to revision of species complexes based on the example of European Phoxinus (Cyprinidae)

Fig. 2

Phylogenetic reconstruction and haplotype network for revision of the genus Phoxinus. Figure 2a Phylogenetic tree constructed from the barcoding region of mitochondrial gene cytochrome oxidase I (COI). Collapsed alignment includes 139 unique haplotypes. The tree was created using Bayesian inference (BI) with BEAST 1.8.0 [26]. Branches carry posterior probabilities (PP) and bootstraps (BS) from the tree constructed with the Maximum-Likelihood (ML) method (PhyML; [29]). Weakly supported nodes are grey and only PP above 0.9 are shown for the main clades (no subclades). -, denotes lack of bootstraps originating from the difference between the BEAST and ML trees. Figure 2b Phylogenetic tree constructed with two partitions: COI and cytochrome b. Collapsed alignment includes 162 unique haplotypes. As in Fig. 2a , BI and ML were used. Because PhyML does not support partitions, GARLI v.2.01 [30, 31] was used for ML. Figure 2c An unrooted minimum-spanning network was constructed with COI using the median joining algorithm [32] implemented in Network 5.0 (www.fluxus-engineering.com) with default settings. The position of the type material in the network is denoted with TYPE PM for Phoxinus marsilii and TYPE PC for P. csikii

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