Environmental isolation explains Iberian genetic diversity in the highly homozygous model grass Brachypodium distachyon

Table 1 Sampled populations of Brachypodium distachyon sorted by geographical area

Locality	Code	N	Latitude (N)	Longitude (W)	Altitude (m.a.s.l)*	H_o	H_e	N_A	A_R	F_IS	s	Exclusive genotypes	pH
NW Spain
Ourense, Sobrado	SOB	9	42.53167	−6.85194	464	0.000	0.000	10	1.000	-	-	1 (11.1%)	5.36
Leon, Campohermoso	CMH	10	42.85472	−5.43472	1074	0.000	0.000	10	1.000	-	-	1 (10.0%)	6.67
Valladolid, Iscar	ISC	8	41.37	−4.53833	828	0.000	0.000	10	1.000	-	-	1 (12.5%)	6.67
NE Spain
Navarra, Lumbier, Foz	LUM	10	42.63651	−1.30484	434	0.000	0.000	10	1.000	-	-	1 (10.0%)	8.01
Navarra, Puerto del Perdon	PER	10	42.73703	−1.74952	736	0.000	0.000	10	1.000	-	-	1 (10.0%)	8.01
Huesca, Sariñena Laguna	SAR	10	41.78622	−0.18278	292	0.000	0.032	11	1.100	1.000	1.000	2 (20.0%)	8.36
Huesca, Yaso, Sierra de Guara	YAS	10	42.2024	−0.12232	731	0.000	0.032	11	1.100	1.000	1.000	2 (20.0%)	8.36
Huesca, Jaca, Banaguas	BAN	10	42.58063	−0.5799	822	0.000	0.018	11	1.100	1.000	1.000	2 (20.0%)	8.36
Huesca, Aren	ARE	10	42.256944	0.72814	681	0.000	0.060	12	1.200	1.000	1.000	3 (30.0%)	8.36
Huesca, Abizanda, Barranco Mallo	ABI	10	42.195	−0.207	382	0.000	0.018	11	1.100	1.000	1.000	2 (20.0%)	8.36
Zaragoza, Candasnos	CAN	9	41.4651	0.0176	206	0.033	0.104	13	1.300	0.711	0.831	5 (55.5%)	8.27
Lleida, Castillo de Mur	MUR	10	42.09763	0.8775	487	0.000	0.018	11	1.100	1.000	1.000	2 (20.0%)	7.87
S Spain
Cadiz, Grazalema	GRAZ	8	36.75583	−5.44167	1103	0.000	0.022	11	1.100	1.000*	1.000	2 (25.0%)	8.09
Almeria, Playazo-Rodalquilar	PLA	13	36.8609867	2.0007	11	0.000	0.014	11	1.100	1.000*	1.000	2 (15.4%)	8.59

The location, population code, number of plants genotyped (N), latitude, longitude, altitude, soil pH, mean observed heterozygosity (H _o) and expected heterozygosity (H _e), total number of alleles (N _A), mean allelic richness (A _R), inbreeding coefficient (F_IS), selfing rate (s), and number of exclusive genotypes (%. between parenthesis) are shown. Asterisks indicate F_IS values (range 0–1) deviating from Hardy-Weinberg Equilibrium (HWE). Soil pH values were retrieved from [51]
*m.a.s.l: meters above sea level

ISSN: 2730-7182