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Table 1 Sampling information and high throughout data of (A) 16S ribosomal RNA genes and (B) protein-coding genes of Bathymodiolus mussel’s thiotrophic endosymbionts, and (C) Bathymodiolus samples examined in this study

From: Geographical structure of endosymbiotic bacteria hosted by Bathymodiolus mussels at eastern Pacific hydrothermal vents

(A)

Vent

Dive loga

Number of host individuals

Count of reads

9°N

A2498, A3540

12

14,260

7°S

A3320

3

2178

11°S

A3323

8

8104

17°S

A3327

7

7024

23°S

A4096

2

2027

32°S

A3339, A3340

9

6649

38°S

A4091

4

3179

(B)

Vent

Dive log

MID sequencesb

Number of host individuals

Count of reads

11°N

A2226

ACGTAGATCGT

8

93,943

9°N

A3540

ACTACGTCTCT

8

105,970

GAR

A2223

ACGCGTCTAGT

8

82,618

7°S

A3320

ACTATACGAGT

8

73,839

11°S

A3323

ACTCGCGTCGT

8

75,903

17°S

A3327

ACACGTAGTAT

8

99,215

23°S

A4096

ACACTACTCGT

8

46,566

32°S

A3340

ACGACACGTAT

8

117,794

38°S

A4091

ACGAGTAGACT

8

92,461

(C)

Vent locality

Latitude

Longitude

Depth (m)

Dive log

Date

Galápagos Rift (GAR)

0°47.9′N

86°09.2′W

2486

A2223

May 28, 1990

East Pacific Rise (EPR)

11°N

11°24.9′N

103°47.3′W

2515

A2226

May 4, 1990

9°N

9°50.5′N

104°17.5′W

2525

A2498

Mar 6, 1992

 

9°49.4′N

104°17.7′W

2518

A3540

Apr 14, 2000

7°S

7°25.0′S

107°48.6′W

2747

A3320

Dec 23, 1998

11°S

11°18.2′S

110°31.8′W

2669

A3323

Dec 27, 1998

17°S

17°24.9′S

113°12.2′W

2578

A3327

Dec 31, 1998

23°S

23°32.9′S

115°34.2′W

2595

A4096

Apr 1, 2005

Pacific-Antarctic Ridge (PAR)

32°S

31°09.4′S

111°55.9′W

2332

A3339

Jan 15, 1999

 

31°51.8′S

112°02.8′W

2331

A3340

Jan 16, 1999

38°S

37°40.4′S

110°52.6′W

2236

A4091

Mar 25, 2005

  1. aHuman-occupied deep-sea vehicle (HOV) Alvin dive numbers
  2. bMultiplex Identifier sequences used for labeling of different populations for pooled 454-pyrosequencing