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Table 3 Results of morphospecies-specific multiplex PCR screening of post-emergence Cardiaspina and Spondyliaspis mummies

From: Unravelling mummies: cryptic diversity, host specificity, trophic and coevolutionary interactions in psyllid – parasitoid food webs

Host
species
Emerged
parasitoid species
Emerged
species DNA
Psyllid
DNA
Other
species DNA
Informative
mummies
Uninformative
mummies
Detection
success (%)
C. albitextura P1 Yes Yes No 20 40 33
P2 female Yes Yes No 7 3 70
P2 male Yes Yes No 2 0 100
H female Yes Yes P2 20 5 80
H male Yes Yes P2 15 2 88
HH female Yes Yes No 5 5 50
HH male
C. fiscella P1 Yes Yes No 0 1 0
P2 female Yes Yes No 2 0 100
P2 male Yes Yes No 2 0 100
H female Yes Yes P2 20 7 74
H male Yes Yes P2 20 6 77
HH female Yes Yes No 8 1 89
HH male Yes Yes P1 4 0 100
C. maniformis P1 Yes Yes No 2 10 17
P2 female Yes Yes P2 17 4 81
P2 male Yes Yes P2 20 3 87
H female
H male
HH female Yes Yes No 8 2 80
HH male Yes Yes P1 4 2 67
C. tenuitela P1 Yes Yes No 8 45 15
P2 female
P2 male
H female Yes Yes P2 14 0 100
H male Yes Yes P2 4 2 67
HH female Yes Yes No 5 3 63
HH male
GB Cardiaspina sp. P1 Yes Yes No 20 162 11
P2 female Yes Yes No 20 5 80
P2 male Yes Yes No 20 4 83
H female Yes Yes P2 20 6 77
H male Yes Yes P2 20 6 77
HH female Yes Yes No 8 1 89
HH male Yes Yes P1 3 7 30
Spondyliaspis sp. P1
P2 female Yes Yes No 3 6 33
P2 male Yes Yes No 4 0 100
H female
H male Yes Yes P2 2 1 67
HH female Yes Yes No 4 1 80
HH male Yes Yes P1 2 0 100
  1. Uninformative mummies were PCR negative for either the psyllid host or the emerging parasitoid. For P1, only a female morphotype was found across all host species. Similarly for HH of C. albitextura and C. tenuitela, only a female morphotype was found. It was therefore concluded that P1 for all host species and HH from C. albitextura and C. tenuitela were thelytokous. HH from C. albitextura and C. tenuitela is likely a cryptic species that is distinct from other HH morphospecies