Fig. 5

Phylogeny of Eleotridae, based on four mitochondrial (cytb, COI, ND1, ND2) genes and calibrated with minimum date of closure of the Isthmus of Panama. Error bars indicate 95% highest posterior density, and black circles at nodes indicate 95–100% posterior probability. Familial classification is indicated on clades, and colored bars indicate ranges: Caribbean = red, Eastern Pacific = green, Indo-Pacific = purple. Black boxes at nodes indicate separation of lineages by the Isthmus of Panama. In the Eleotris clade, other geminate splits than the one examined here have occurred, but were not included in this analysis