Fig. 6
From: Human MFAP1 is a cryptic ortholog of the Saccharomyces cerevisiae Spp381 splicing factor
Minimal scPrp38-scSpp381 and scSnu23-scPrp38-scSpp381 complexes resemble analogous MFAP1-based complexes in H. sapiens and C. thermophilum. Coomassie Blue-stained SDS-PAGE gels and corresponding chromatograms of analytical gel filtration experiments with the indicated scPrp38 and scSpp381 (a), or with the indicated scPrp38, scSpp381 and scSnu23 variants (b). Experiments in (a) were performed with a Superdex 200 increase 3.2/300 column. Experiments in (b) were carried out on a Superdex 75 10/300 column (both GE Healthcare). M – marker; L – load; horizontal black lines – fractions analyzed by SDS-PAGE