Fig. 5
From: Human MFAP1 is a cryptic ortholog of the Saccharomyces cerevisiae Spp381 splicing factor
The Prp38-MFAP1/Spp381 interface is conserved between yeast and human. Coomassie Blue-stained SDS-PAGE gels and corresponding chromatograms of analytical gel filtration experiments with full-length scPrp38 and scSpp381. a Wild type proteins. b Wild type scSpp381 in combination with the scPrp38 D189A variant or wild type scPrp38 in combination with the scSpp381 R192A variant. All experiments were performed with a Superdex 75 3.2/30 column (GE Healthcare). M – marker; L – load; horizontal black lines – fractions analyzed by SDS-PAGE