Fig. 3

Functional prediction of important substitutions identified from giraffe TLR3. a A TLR3 cladogram (maximum likelihood) demarcating terrestrial ungulates (black) and cetacean (green) branches used in the clade analysis of functional divergence. b Crystal structure of human TLR3 ECD (PDB ID: 2A0Z) showing sites corresponding to giraffe key substitutions based on whether they are predicted to change function (PolyPhen), positive selection sites or map to empirically important site on the structure. c Partial alignment to show residues in other species at sites corresponding to giraffe important sites (❶ denotes TLR3 N-glycosylated site, ❷ show PolyPhen hit site and ❸ show positive selected sites based on PAML and SLR). Top number lane represents the residue position for human TLR and the second number lane is the residue position in the alignment. The asterisk (*) refers to residue identical to that of giraffe