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Table 2 Results from PERMANOVA and ANOSIM analysis of one-week-old F2-juveniles per functional gene categories

From: Grandparental immune priming in the pipefish Syngnathus typhle

F2-juveniles (One-week-old) Immune genes [29 total] Innate genes [13] Adaptive genes [8] Innate & Adaptive genes [5] Complement component genes [3] Epigenetic genes [15 total] DNA-methylation genes [5] Histone-de/methylation genes [4] Histone deacetlyation genes [3] Histone acetylation genes [2]
F0-sex (DF = 3) <0.001 0.004 ns 0.001 0.017 0.035 ns ns ns 0.035
ANOSIM-Global R 0.115 0.12 ns 0.104 0.054 0.088 ns ns ns 0.088
Significance level 0.1% 0.1% ns 0.1% 0.1% 0.1% ns ns ns 0.1%
F0-Bi, F0-Mat 0.004 0.001 ns 0.003 ns 0.027 ns ns ns 0.008
F0-Bi, F0-Pat 0.003 ns ns 0.002 0.009 ns ns ns ns ns
F0-Mat, F0-Pat 0.007 0.001 ns 0.001 ns 0.001 ns ns ns 0.001
F0-Bi, F0-N 0.001 0.001 ns 0.001 0.002 0.001 ns ns ns 0.009
F0-Mat, F0-N 0.001 0.001 ns 0.002 0.002 0.009 ns ns ns ns
F0-Pat, F0-N 0.001 0.001 ns 0.001 ns 0.001 ns ns ns 0.007
F2-bacteria (DF = 2) <0.001 0.026 ns <0.001 0.001 0.030 0.022 ns ns 0.019
ANOSIM-Global R 0.022 0.018 ns 0.026 0.024 0.004 0.011 ns ns 0.009
Significance level 7.4% 11.5% ns 2.1% 5.9% 31.9% 20.9% ns ns 72.9%
F2-V+, F2-T+ ns ns ns ns ns ns ns ns ns ns
F2-V+, F2-N 0.022 0.011 ns 0.006 0.024 0.005 0.005 ns ns ns
F2-T+, F2-N 0.021 0.016 ns 0.030 ns 0.049 0.049 ns ns 0.05
F0-sex x F2-bacteria (DF = 6) 0.009 0.007 ns ns ns 0.029 ns ns ns ns
ANOSIM-Global R 0.105 0.103 ns ns ns 0.074 ns ns ns ns
Significance level 0.1% 0.1% ns ns ns 0.1% ns ns ns ns
F0-Mat/F2-V+, F0-Pat/F2-V+ 0.036 0.030 ns ns ns 0.004 ns ns ns ns
F0-Mat/F2-V+, F0-Pat/F2-T+ 0.049 0.007 ns ns ns 0.004 ns ns ns ns
F0-Mat/F2-T+, F0-Pat/F2-V+ 0.025 0.028 ns ns ns 0.004 ns ns ns ns
F0-Mat/F2-T+, F0-Pat/F2-T+ 0.033 0.04 ns ns ns 0.010 ns ns ns ns
For further details see Additional file 1 : Table S2
  1. Multivariate ANOSIM was performed following significant PERMANOVA effects to assess differences in the gene expression profiles per treatment groups applying pairwise comparison on relative gene expression data (−∆Ct-values) based on a Euclidean distance matrix and 10000 permutations. Pairwise comparison was conducted for following two fixed factors and their interactions: ‘F0-sex’ (grandparental (F0-Bi), grand-maternal (F0-Mat), grand-paternal (F0-Pat), grandparental control (F0-N)) and ‘F2-bacteria’ (F2-offspring control (F2-N), F2-offspring Vibrio (F2-V+) and Tenacibaculum (F2-T+) bacteria treatment)