Grandparental immune priming in the pipefish Syngnathus typhle

Beemelmanns, Anne; Roth, Olivia

doi:10.1186/s12862-017-0885-3

BMC Ecology and Evolution

Table 2 Results from PERMANOVA and ANOSIM analysis of one-week-old F2-juveniles per functional gene categories

From: Grandparental immune priming in the pipefish Syngnathus typhle

F2-juveniles (One-week-old)	Immune genes [29 total]	Innate genes [13]	Adaptive genes [8]	Innate & Adaptive genes [5]	Complement component genes [3]	Epigenetic genes [15 total]	DNA-methylation genes [5]	Histone-de/methylation genes [4]	Histone deacetlyation genes [3]	Histone acetylation genes [2]
F0-sex (DF = 3)	<0.001	0.004	ns	0.001	0.017	0.035	ns	ns	ns	0.035
ANOSIM-Global R	0.115	0.12	ns	0.104	0.054	0.088	ns	ns	ns	0.088
Significance level	0.1%	0.1%	ns	0.1%	0.1%	0.1%	ns	ns	ns	0.1%
F0-Bi, F0-Mat	0.004	0.001	ns	0.003	ns	0.027	ns	ns	ns	0.008
F0-Bi, F0-Pat	0.003	ns	ns	0.002	0.009	ns	ns	ns	ns	ns
F0-Mat, F0-Pat	0.007	0.001	ns	0.001	ns	0.001	ns	ns	ns	0.001
F0-Bi, F0-N	0.001	0.001	ns	0.001	0.002	0.001	ns	ns	ns	0.009
F0-Mat, F0-N	0.001	0.001	ns	0.002	0.002	0.009	ns	ns	ns	ns
F0-Pat, F0-N	0.001	0.001	ns	0.001	ns	0.001	ns	ns	ns	0.007
F2-bacteria (DF = 2)	<0.001	0.026	ns	<0.001	0.001	0.030	0.022	ns	ns	0.019
ANOSIM-Global R	0.022	0.018	ns	0.026	0.024	0.004	0.011	ns	ns	0.009
Significance level	7.4%	11.5%	ns	2.1%	5.9%	31.9%	20.9%	ns	ns	72.9%
F2-V+, F2-T+	ns	ns	ns	ns	ns	ns	ns	ns	ns	ns
F2-V+, F2-N	0.022	0.011	ns	0.006	0.024	0.005	0.005	ns	ns	ns
F2-T+, F2-N	0.021	0.016	ns	0.030	ns	0.049	0.049	ns	ns	0.05
F0-sex x F2-bacteria (DF = 6)	0.009	0.007	ns	ns	ns	0.029	ns	ns	ns	ns
ANOSIM-Global R	0.105	0.103	ns	ns	ns	0.074	ns	ns	ns	ns
Significance level	0.1%	0.1%	ns	ns	ns	0.1%	ns	ns	ns	ns
F0-Mat/F2-V+, F0-Pat/F2-V+	0.036	0.030	ns	ns	ns	0.004	ns	ns	ns	ns
F0-Mat/F2-V+, F0-Pat/F2-T+	0.049	0.007	ns	ns	ns	0.004	ns	ns	ns	ns
F0-Mat/F2-T+, F0-Pat/F2-V+	0.025	0.028	ns	ns	ns	0.004	ns	ns	ns	ns
F0-Mat/F2-T+, F0-Pat/F2-T+	0.033	0.04	ns	ns	ns	0.010	ns	ns	ns	ns
For further details see Additional file 1 : Table S2

Multivariate ANOSIM was performed following significant PERMANOVA effects to assess differences in the gene expression profiles per treatment groups applying pairwise comparison on relative gene expression data (−∆Ct-values) based on a Euclidean distance matrix and 10000 permutations. Pairwise comparison was conducted for following two fixed factors and their interactions: ‘F0-sex’ (grandparental (F0-Bi), grand-maternal (F0-Mat), grand-paternal (F0-Pat), grandparental control (F0-N)) and ‘F2-bacteria’ (F2-offspring control (F2-N), F2-offspring Vibrio (F2-V+) and Tenacibaculum (F2-T+) bacteria treatment)

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