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Table 1 Results from 2-way PERMANOVA analysis of gene expression of one-week-old F2-juveniles

From: Grandparental immune priming in the pipefish Syngnathus typhle

Gene categories Model F0-sex F2-bacteria F0-sex x F2-bacteria Size
R2 F.Model Pr(>F)   F.Model Pr(>F)   F.Model Pr(>F)   F.Model Pr(>F)
Immune genes [29 genes-total] 0.83 6.82 > 0.001 *** 3.08 >0.001 *** 1.32 0.009 ** 1.13 0.641
 Innate immune genes [13 genes] 0.83 6.67 0.004 ** 2.01 0.026 * 1.87 0.007 ** 1.26 0.431
 Adaptive immune genes [8 genes] 0.80 1.53 0.108   1.00 0.184   0.99 0.100   1.71 0.521
 Innate & Adaptive genes [5 genes] 0.84 5.88 0.001 ** 5.47 >0.001 *** 0.71 0.622   0.86 0.460
 Complement component genes [3 genes] 0.86 5.31 0.017 * 3.66 0.001 *** 0.80 0.237   0.71 0.790
Epigenetic genes [15 genes-total] 0.85 6.63 0.035 * 1.64 0.030 * 1.22 0.029 * 0.62 0.894
 DNA-methylation genes [5 genes] 0.85 6.09 0.061 . 2.26 0.022 * 1.18 0.081 . 0.84 0.812
 Histone de/methylation genes [4 genes] 0.89 4.16 0.195   0.68 0.516   1.33 0.082 . 0.20 0.844
 Histone deacetylation genes [3 genes] 0.86 5.65 0.079 . 1.23 0.126   1.21 0.060 . 1.39 0.621
 Histone acetylation genes [2 genes] 0.78 12.47 0.035 * 2.03 0.019 * 1.09 0.065 . 0.15 0.896
Degrees of Freedom   DF = 3 DF = 2 DF = 6 DF = 1
Residual Degrees of Freedom 174  
Total Degrees of Freedom 186 For further details see Additional file 1 : Table S1
  1. Multivariate PERMANOVA analysis to assess the effect and interaction of the two fixed factors F0-sex and F2-offspring, size as covariate and family as strata term on relative gene expression values (−∆Ct-values). Each analysis was based on an Euclidean distance matrix with p-values obtained by 10000 permutations. Significant p-values are marked in bold letters and asterix symbol (significance code: <0.001***, 0.001**, 0.01*, 0.1 > p-value ≥ 0.05 trend ). R2 value indicate the percentage of variance explained by the model