Fig. 3

Genomes of the TEV-eGFP lineages evolved the three different hosts as compared to the ancestral lineage. Mutations were detected using NGS data of the evolved lineages (L1-L10), as compared to their ancestral population. The square symbols represent mutations that are fixed (>50%) and the circle symbols represent mutations that are not fixed (<50%). Filled symbols represent nonsynonymous substitutions and open symbols represent synonymous substitutions. The triangle symbols represent mutations that are present in either the 3’UTR or 5’UTR. Black substitutions occur only in one lineage, whereas color-coded substitutions are repeated in two or more lineages. Note that the mutations are present at different frequencies as reported by VarScan 2. Grey boxes with continuous black lines indicate genomic deletions in the majority variant of the virus population. The grey transparent box with dotted black lines in L4 of N. benthamiana indicates a genomic deletion in a minority variant. The latter box was drawn to indicate the size of the deletion, assuming that the deletion starts at the first position of eGFP. The mutations discussed in the manuscript are highlighted on the top. For more information on the frequency of the mutations please see Additional file 2: Tables S1-S3