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Fig. 6 | BMC Evolutionary Biology

Fig. 6

From: Mid-day siesta in natural populations of D. melanogaster from Africa exhibits an altitudinal cline and is regulated by splicing of a thermosensitive intron in the period clock gene

Fig. 6

SNP3/A flies exhibit a larger altitudinal effect on mid-day sleep compared to the SNP3/G variant. a Shown are the group averages of median sleep bout length during the mid-day (ZT3-9) for flies with SNP3/A (blue dot and line), SNP3/G (red dot and line), or the combination of all 91 lines tested (black dot and line), as a function of altitude in 1000 m increments. The results shown are based on the same activity data used to generate Fig. 5, except that for SNP3/A and SNP3/G flies we only used data from the 67 independent lines where the identity of the SNP3 variant was known (see Additional file 1: Table S1). Briefly, flies were exposed to at least 5 days of LD at 25 °C and activity data from the last three days of LD was pooled. The lines in the panel represent the regression analysis of phenotypic means (y-axis) as a function of altitude (x-axis). b-d Shown are group averages of median sleep bout length during the mid-day (ZT3-9) for flies from Cameroon and Kenya that were exposed to at least 5 days of LD at the indicated temperature (top of panels). The results are based on the same flies used in Fig. 1; listed according to altitude group and SNP variant the flies analyzed were; low altitude SNP3/A (CM16, KM10, KM16, KM20); high altitude SNP3/A (CO4, CO8, CO16, KN5, KN6M, KN11M, KN19M, KO2, KO6, KO10M); low altitude SNP3/G (CM17, CM22, CM54, CY1); high altitude SNP3/G (CO1, CO2, CO10, CO13, CO15, KN13M, KN23M). Values for SNP3/A and SNP3/G flies are significantly different using student’s t-test; *, p < 0.05; **, p < 0.01

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