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Fig. 5 | BMC Evolutionary Biology

Fig. 5

From: Mid-day siesta in natural populations of D. melanogaster from Africa exhibits an altitudinal cline and is regulated by splicing of a thermosensitive intron in the period clock gene

Fig. 5

Altitudinal cline in daytime sleep behavior is widely observed in flies from tropical Africa. a-f Shown are group averages of total sleep (a-b), median sleep bout length (c-d), and number of sleep bouts (e-f) during the 12-h period of either daytime or nighttime in LD for a total of 91 independent lines (16 flies per line) from 20 localities in 10 different countries (see Additional file 1: Table S1). Flies were exposed to at least 5 days of LD at 25 °C and activity data from the last three days of LD was pooled and analyzed in 1000 m increments. The lines in the panels represent the regression analysis of phenotypic means (y-axis) as a function of altitude (x-axis). The results show that there is a more pronounced altitudinal cline in daytime sleep levels and quality compared to nighttime sleep, although the daytime trend of more fragmented daytime sleep with increasing altitude is still observed with nighttime sleep

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