Fig. 3
Dark field imagery of 36 h embryos. (a, d1) An R3 × R3 larval cuticle just before hatching (pre-eclosure), T2 arrow indicates 2nd thoracic spiracle, A1 arrow indicates 1st abdominal spiracle, A10 arrow indicates 10th abdominal deticle belt. (b, d2) a representative unhatched 13.2 × R3 cuticle at two focal planes. (c, d2’) An unhatched 13.2 × R3 cuticle at two focal planes, closed arrow in indicates the edge of cuticle bordering the dorsal hole indicating a failure in dorsal closure