Table 1 List of the primers used for gene expression analysis by quantitative real-time PCR in sea bream (Sparus aurata) skin
From: Evolution of the angiopoietin-like gene family in teleosts and their role in skin regeneration
Symbol | Accession Number | Primer sequences (5’to 3’) | Amplicon (bp) | T (°C) | Efficiency (%) | R2 |
|---|---|---|---|---|---|---|
angptl1b | Â | F: GCATGCAGGTCTACAGTCG R: CAAAGGCTCGGGTGTTGTC | 135 | 58 | 96 | 0.99 |
angptl2b | Â | F: TGCTGCACGAGATCATCAGGAA R: GTACTTGTGCTCGAGATCTTT | 128 | 60 | 89 | 0.99 |
angptl4a | Â | F: AGATACAGAAGGCTGATGCT R: CTGGTCGTTGTCTTGGTC | 101 | 60 | 99 | 0.99 |
angptl4b | Â | F: AAATAATGTCGACCGAAGAG R: CGAGTTACCACAGCTGTTG | 128 | 60 | 81 | 0.99 |
angptl7 | Â | F: CAGTACGCTCAGGATCGAGATGG R: ATGGTGCTGAAGTTGGTGTTGTT | 171 | 60 | 97 | 0.99 |
vegfab | Â | F: ACGTCCAGCTATAACATTACAA R: CTTTCTTTAACCTACACTCA | 115 | 58 | 90 | 0.99 |
rps18 | AM490061 | F: AGGGTGTTGGCAGACGTTAC R: CTTCTGCCTGTTGAGGAACC | 164 | 60 | 88 | 0.99 |
ß-actin | X89920 | F: CCCTGCCCCACGCCATCC R: TCTCGGCTGTGGTGGTGAAGG | 94 | 60 | 92 | 0.99 |