Evidence for genetic variation in Natterer’s bats (Myotis nattereri) across three regions in Germany but no evidence for co-variation with their associated astroviruses

Table 1 Genetic diversity at nuclear loci within Myotis nattereri populations sampled within Germany

	Final concentration [μM]	BY					NRW					MV
	Final concentration [μM]	N	A	H _e	H _o	Ar	N	A	H _e	H _o	Ar	N	A	H _e	H _o	Ar
Multiplex 1
A2-Mluc	0.22	92	11	0.78	0.76	10.93	100	10	0.63	0.63	9.72	168	13	0.82	0.82	12.50
b22	0.22	92	7	0.75	0.78	6.94	100	8	0.78	0.74	7.70	172	8	0.79	0.77	6.99
D15	0.22	92	10	0.87	0.92	10.00	100	10	0.86	0.86	10.00	172	11	0.80	0.85	10.45
EF15	0.44	92	4	0.51	0.41	4.00	100	4	0.59	0.51	3.86	172	5	0.64	0.62	4.88
G30-Mluc	0.22	92	19	0.88	0.90	18.86	97	20	0.83	0.80	19.41	170	22	0.83	0.78	19.10
G6-Mluc	0.22	92	8	0.82	0.78	7.94	100	9	0.82	0.85	8.96	172	9	0.84	0.83	8.75
H23	0.22	92	9	0.80	0.83	8.99	100	11	0.82	0.81	10.86	172	11	0.79	0.80	10.49
H29	0.56	92	6	0.69	0.71	6.00	100	8	0.72	0.67	7.84	172	8	0.68	0.63	6.63
Mnatt-1	0.11	91	7	0.70	0.74	6.94	100	5	0.64	0.69	5.00	172	7	0.69	0.68	6.63
Mnatt-11*	0.56	91	8	0.83	0.85	7.94	100	9	0.81	0.78	8.70	172	10	0.85	0.88	9.47
Mnatt-8	0.56	86	19	0.90	0.88	19.00	99	25	0.93	0.88	24.56	172	26	0.93	0.85	22.31
Multiplex 2
A13-Mluc	0.44	89	16	0.85	0.91	15.87	100	16	0.83	0.80	15.58	172	14	0.88	0.90	13.74
E5-Mluc	1.06	87	10	0.80	0.78	10.00	100	13	0.81	0.76	12.70	172	12	0.81	0.80	10.97
FV5AP	0.22	91	4	0.68	0.59	4.00	100	5	0.65	0.63	5.00	172	7	0.70	0.75	6.44
G31-Mluc	0.22	92	6	0.80	0.79	6.00	99	8	0.81	0.81	7.87	172	8	0.79	0.79	7.75
GZBYR	0.22	92	9	0.78	0.74	9.00	100	10	0.83	0.78	9.86	171	10	0.83	0.81	9.86
H19	0.33	92	18	0.87	0.83	17.93	100	15	0.89	0.88	14.70	171	17	0.83	0.78	15.90
Mnatt-2*	0.22	92	8	0.83	0.85	8.000	100	9	0.81	0.78	8.70	170	9	0.85	0.86	8.97
Mschreib3	0.22	87	16	0.90	0.84	15.99	99	17	0.90	0.85	16.60	169	21	0.93	0.86	20.08

N number of samples successfully analyzed (total number of individuals: Bavaria (BY) 92, North Rhine Westphalia (NRW) 100, Mecklenburg Western Pomerania (MV) 172); A number of alleles; H _o observed heterozygosity; H _e expected heterozygosity; (A_R) allelic richness based on a minimum of 74 individuals. The primers marked with an asterisk (*) appear to be linked. Forward markers were dyed as follows: 6-FAM: A13-Mluc, D15, b22, G30-Mluc, H19, H29, GZBYR, FV5AP, Mnatt-1, Mnatt-2; VIC: Mnatt-8, G31-Mluc, H23; NED: A2-Mluc, EF15, Mschreib3, E5-Mluc and PET: Mnatt-11, G6-Mluc

ISSN: 2730-7182