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Table 2 Phylogenetic evidence of HGT in neighbouring genes

From: Horizontal gene transfer drives the evolution of Rh50 permeases in prokaryotes

Species

IMG Locus_tag a

IMG product name

Tree label b

# taxa

# sites

Adjacent taxa in tree

Methanomassiliicoccus luminyensis B10

missing (Mlum65)

NADP oxidoreductase, coenzyme F420-dependent

T01

85

146

Alpha-, Deltaproteobacteria

Ca. Kuenenia stuttgartiensis

kustc0379

Unknown protein

T02

47

368

div. BRC1 bacterium; Gammaproteobacteria

"

kustc0382

Glutamate formimidoyltransferase

T03

85

484

Marine euryarchaeotes

"

kustc0383

Glutaredoxin-like protein

T04

39

74

Firmicutes

"

kustc0384

Permease of the major facilitator superfamily

T05

61

389

Deltaproteobacteria

Planctomycetaceae bacterium KSU-1

missing (KSU_979)

Indole-3-glycerol phosphate synthase

T06

74

238

Acidobacteria

"

missing (KSU_981)

Conserved hypothetical protein

T07

63

88

Deltaproteobacteria

Ca. Brocadia anammoxidans WQC04

missing (Brocad1)

Indole-3-glycerol phosphate synthase

see T06

   

"

missing (Brocad3)

Predicted membrane protein

see T07

   

Nitrosomonas europaea ATCC 19718

NE0446

3-demethylubiquinone-9-3-methyltransferase

T08

76

156

Gammaproteobacteria

"

NE0447

3-methyladenine DNA glycosylase I

T09

59

189

Gammaproteobacteria

"

NE0449

Aspartate and glutamate racemase

T10

45

238

Gammaproteobacteria

Nitrosomonas sp. AL212

NAL212_0966

Hypothetical protein

T11

51

141

Gammaproteobacteria

Geobacter sp. M21

GM21_1429

Diguanylate cyclase

T12

55

156

Gammaproteobacteria

  1. Phylogenetic Bayesian inference was carried out in PhyloBayes, ML inference in RAxML and PhyML. Number of taxa and sites in the alignment are given. All BI analyses were run to convergence (maxdiff < 0.1 and eff. size >100). ML in RAxML used “-f a” option with 1,000 rapid-bootstrap replicates. ML in PHyML used SPR tree-space search strategy with 5 random starts + BioNJ. Prottest best-fitting model was LG + Г4 + F for T05, T07, T09, T10, datasets, LG + Г4 for all the others. See Additional file 1: Table S4 for full data
  2. aWhen Integrated Microbial Genomes (IMG) locus tag was missing, an arbitrary one was chosen
  3. bAll trees in Additional file 3