Multi-locus phylogeny using topotype specimens sheds light on the systematics of Niviventer (Rodentia, Muridae) in China

Table 5 Primers and polymerase chain reaction (PCR) cycling conditions used in this study

Genes	Primer Name	Nucleotide sequence 5′ to 3′	Annealing Temperature (°C)	Fragment Length (bp)	Citation
CYT B	L14724_hk3	GGACTTATGACATGAAAAATCATCGTTG	47.5 °C	1140	He et al. 2010 [38]
CYT B	H15915_hk3	TCTCCATTTCTGGTTTACAAGAC	47.5 °C	1140	He et al. 2010 [38]
IRBP	IRBP-217	ATCCCCTATGTCATCTCCTACYTG	60 °C	1149	Stanhope et al. 1992 [39]
IRBP	IRBP-1531	CGCAGGTCCATGATGAGGTGCTCCGTGTCCTG	60 °C	1149	Stanhope et al. 1992 [39]
RAG1	S70	TCCGAGTGGAAATTTAAGMTGTT	51.7 °C	1221	Steppan et al. 2004 [40]
RAG1	S73	GAGGAAGGTRTTGACACGGATG	51.7 °C	1221	Steppan et al. 2004 [40]
GHR	GHR-F	GAGTTCATTGAGCTGGATAT	60 °C	777	Current Study
GHR	GHR-R	ATGAGTTGCGCTGACGA	60 °C	777	Current Study

ISSN: 2730-7182