A core phylogeny of Dictyostelia inferred from genomes representative of the eight major and minor taxonomic divisions of the group

Table 2 Species and culture conditions

Species	Strain/isolate	Medium	Temperature
Acytostelium ellipticum	AE2	1/5^th SM + 0.5% charcoal	21 °C
Acytostelium leptosomum	FG12A	1/5^th SM +0.5% charcoal	25 °C
Dictyostelium deminutivum	MexM19A	1/3^rd LP + 0.5% charcoal	25 °C
Dictyostelium polycarpum	OhioWILDS	1/5^th SM	21 °C
Dictyostelium polycephalum	MY1-1	1/5^th SM	25 °C
Polysphondylium violaceum	P6	SM	21 °C

The species that were used for genome sequencing are listed with their preferred growth conditions. Full SM agar contains 10 g BactoTM peptone, 1 g yeast extract, 10 g glucose, 1 g MgSO₄.7H₂O, 2.2 g KH₂PO₄, 1.25 g Na₂HPO₄.2H₂O and 15 g agar per liter, for 1/5^th SM all quantities, except the agar and phosphates are five-fold reduced. Full LP agar contains 1 g lactose, 1 g BactoTM peptone, 2.2 g KH₂PO₄, 1.25 g Na₂HPO₄.2H₂O and 15 g agar per liter. For 1/3^rd LP the lactose and peptone quantities are three-fold reduced

ISSN: 2730-7182