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Table 1 The host-encoded ETP1 is enriched in the purified endosymbiont fraction. 500 ng tryptic digested protein of each sample (whole cell lysate and purified endosymbionts from three independent experiments), was analyzed by tandem mass spectrometry (see Methods)

From: Development of a toolbox to dissect host-endosymbiont interactions and protein trafficking in the trypanosomatid Angomonas deanei

Protein

Protein IDa

Subcellular localizationb

Averaged MS/MS spectral countsd

Lysate

Endosymbiont

ETP1c

S9VAC8

-

12.3 ± 2.5

27.7 ± 4.0

α-Tubulin

S9WM11

Cytoskeleton

161.7 ± 8.6

7.7 ± 7.2

ATP synthase sub. αe

S9VLV1

Mitochondria

54.3 ± 7.6

5.7 ± 5.1

ATP synthase sub. βe

S9UWU8

Mitochondria

84.7 ± 2.9

11.3 ± 8.5

NADH-FRDf

S9VM53

Mitochondria & glycosomes

30.3 ± 4.2

14.0 ± 12.3

  1. aProtein identifier corresponding to the Uniprot Database (http://www.uniprot.org)
  2. b Subcellular localization found in other trypanosomatid species
  3. cEndosymbiont-targeted protein 1
  4. dAverage and standard deviations were calculated from three independent experiments
  5. eSubunit α and β of the mitochondrial ATPase complex
  6. fNADH-dependent fumarate reductase