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Fig. 6 | BMC Evolutionary Biology

Fig. 6

From: Development of a toolbox to dissect host-endosymbiont interactions and protein trafficking in the trypanosomatid Angomonas deanei

Fig. 6

Host-encoded ETP1 is specifically targeted to the β-proteobacterial endosymbiont in A. deanei. (a) pAEX-EGFP-ETP1 and pAEX-ETP1-EGFP plasmids for expression of ETP1 fused to the C- and N-terminus of EGFP, respectively. Brown rectangle or arrow, ETP1. Remaining color code and abbreviations are as in Fig. 5. (b) Cells expressing the N- or C-terminal ETP1-EGFP fusion protein were analyzed by FISH-IFA using a 5′-Cy3 labeled probe against the bacterial 16S rRNA and an α-GFP IgG as described in Methods. EGFP-ETP1, N-terminal fusion of EGFP; ETP1-EGFP, C-terminal fusion of EGFP; ES, eight-shaped endosymbiont; K, kinetoplast; N, nucleus. Scale bar: 2 μm

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