Fig. 1

RIG-like receptors (RLRs) arose in early animals and diversified by gene duplications in bilateria and jawed vertebrates. We reconstructed the RLR gene phylogeny by maximum likelihood using two different alignments of all available RLR protein sequences (see Methods). Branch lengths are scaled to the average number of substitutions/site. We plot SH-like aLRT clade support from PROBALIGN (top) and MAFFT (bottom) alignments; clades with <0.8 support are collapsed to polytomies. Sequences from major monophyletic taxonomic groups are collapsed, and genbank IDs are provided for sequences from individual species. Bold red branches indicate significant support for protein-coding adaptation specific to the RNA-recognition (RD) domain (p < 0.01 after correction for multiple tests; see Methods). White circles indicate ancestral proteins resurrected in this study