Comparative phylogeography in the Atlantic forest and Brazilian savannas: pleistocene fluctuations and dispersal shape spatial patterns in two bumblebees

Table 1 Characteristics of the mitochondrial regions analyzed for Bombus morio (Bm) and B. pauloensis (Bp)

Mitochondrial region	sp.	Sequence size (bp)	Variable sites	Frequency (%)				h	Hd	Model	Primers pairs
Mitochondrial region	sp.	Sequence size (bp)	Variable sites	A	C	G	T	h	Hd	Model	Primers pairs
COI	Bm	399	26 (6.5%)	30.6	13.6	10.2	45.6	26	0.7607	TPM1uf + I + G	mtD6 and mtD9 [103]
COI	Bp	399	17 (4.3%)	30.8	13.6	10.4	45.3	25	0.7846	TIM1 + I	mtD6 and mtD9 [103]
CytB	Bm	396	24 (6.1%)	34.9	12.8	7.6	44.7	33	0.8686	GTR + G	AMB16 [104] and mtD26 [103]
CytB	Bp	396	21 (5.3%)	30.0	12.8	6.2	43.0	26	0.8202	HKY + I	AMB16 [104] and mtD26 [103]
16S	Bm	378	10 (2.6%)	42.0	6.9	11.7	39.4	11	0.2709	HKY + I	Cox2 and Atp8rev [105]
16S	Bp	378	7 (1.9%)	40.2	7.5	11.7	40.7	8	0.1388	TPM1uf	Cox2 and Atp8rev [105]
Cl4	Bm	397	24 (6%)	40.2	9.8	11.0	39.0	22	0.6735	TrN + I	16SF and 16SR [103]
Cl4	Bp	396	24 (6.1%)	40.7	10.4	9.9	39.1	29	0.8850	TrN + G	16SF and 16SR [103]
Concatenated	Bm	1570	86 (5.4%)	36.8	10.8	10.1	42.2	94	0.9677	-	-
Concatenated	Bp	1569	71 (4.5%)	37.3	11.1	9.5	42.1	72	0.9501	-	-

COI: Cytochrome C oxidase I; CytB: Cytochrome B; 16S: large ribosomal RNA subunit; Cl4: cluster 4 of tRNA (covering a region of COII and ATPase 8 genes and tRNA^lys and tRNA^asp); sp.: species; h: number of haplotypes; Hd: haplotype diversity; Model: nucleotide substitution model

ISSN: 2730-7182