Spatial pattern of genetic diversity and selection in the MHC class II DRB of three Neotropical bat species

Table 1 PCR primers used to amplify MHC class II DR beta loci in the three species investigated: C. perspicillata, D. rotundus and M. molossus

Primers	Code^a	Position^a	5′ to 3′ Sequence	Designed for	Source
BatCMHIIF	F	Exon 1	GATHCTGTTGGYACTGAGC	D. rotundus	This study
BatCMHIIF.1	F1	Exon 1	TGGTGATMCTGTTGGCACTGAGC	Bats	This study
EX1Fd^b	X1	Exon 1	CTGWTGGYACTGAGCCCTCYCCTGGCT	Myotis sp.	Richman et al. [39]
JSex1.5-DRB	JS1	Exon 1	KGGGCCAGRGRCACACCA	Bats	Schad et al. [35]
JS2Cape-DRB	JS2	Exon 2	AGTGTCAKTWCTCCAACSGGAC	C. perspicillata	Schad et al. [35]
EX3Rd^b	X3	Exon 3	CAGSAGGTTGTGGTGCTGCAG	Myotis sp.	Richman et al. [39]
BatCMHIIR	R	Exon 4	TTCAGACTGYGCCCTCCAYT	D. rotundus	This study
BatCMHIIR.1	R1	Exon 4	CAGACTGYGCCCTCCAYTCCA	Bats	This study
DRB-L729	L729	Exon 4	ACTCAMCATCTTGCTCTG	Mammals	Bowen et al. [50]

^asee Additional file: Figure S2, ^bPrimer modified from Richman et al. [39] to amplify the exon 2 of MHC class II DRB gene

ISSN: 2730-7182