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Table 1 PCR primers used to amplify MHC class II DR beta loci in the three species investigated: C. perspicillata, D. rotundus and M. molossus

From: Spatial pattern of genetic diversity and selection in the MHC class II DRB of three Neotropical bat species

Primers Codea Positiona 5′ to 3′ Sequence Designed for Source
BatCMHIIF F Exon 1 GATHCTGTTGGYACTGAGC D. rotundus This study
BatCMHIIF.1 F1 Exon 1 TGGTGATMCTGTTGGCACTGAGC Bats This study
EX1Fdb X1 Exon 1 CTGWTGGYACTGAGCCCTCYCCTGGCT Myotis sp. Richman et al. [39]
JSex1.5-DRB JS1 Exon 1 KGGGCCAGRGRCACACCA Bats Schad et al. [35]
JS2Cape-DRB JS2 Exon 2 AGTGTCAKTWCTCCAACSGGAC C. perspicillata Schad et al. [35]
EX3Rdb X3 Exon 3 CAGSAGGTTGTGGTGCTGCAG Myotis sp. Richman et al. [39]
BatCMHIIR R Exon 4 TTCAGACTGYGCCCTCCAYT D. rotundus This study
BatCMHIIR.1 R1 Exon 4 CAGACTGYGCCCTCCAYTCCA Bats This study
DRB-L729 L729 Exon 4 ACTCAMCATCTTGCTCTG Mammals Bowen et al. [50]
  1. asee Additional file: Figure S2, bPrimer modified from Richman et al. [39] to amplify the exon 2 of MHC class II DRB gene