Fig. 2

Splicing and mobility of the Ef.PcfG intron in its native environment. a Schematic of the group II intron splicing pathway. Position of the primers (Additional file 1: Table S2) used to amplify ligated exons (E1/E2) (black arrows, 1587 bp) and the intron splice junction (open arrows, 287 bp) by RT-PCR is depicted. RT-PCR amplifications of ligated exons b and of intron splice junctions c were performed on total RNA extracts from E. faecalis (SF24397ΔpEF1071). The RT-PCR amplicons corresponding to pcfG ligated exons (b, 1587 bp) and Ef.PcfG spliced junction (c, 287 bp) were excised and directly sequenced. d Mobility efficiency of Ef.PcfG to the relaxase mobA gene (E1/E2) on pEF1071. Position of the primers (Additional file 1: Table S2) used to amplify the 5’ (black arrows, 626 bp, E1-Ef.PcfG) and 3’ (open arrows, 1201 bp, Ef.PcfG-E2) junctions of Ef.PcfG mobility products in mobA by PCR is depicted