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Fig. 3 | BMC Evolutionary Biology

Fig. 3

From: Huntingtin-associated protein 1: Eutherian adaptation from a TRAK-like protein, conserved gene promoter elements, and localization in the human intestine

Fig. 3

Phylogenetic relationship of HAP1_N domains of selected vertebrates and Drosophila HAP1_N family members. Multiple sequence alignment was performed with the ClustalW program. The phylogenetic tree was constructed using the neighbor-joining method. The tree was rooted using the Drosophila Milton HAP1_N domain sequence as an outgroup. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) is shown next to the branches, where equal to or greater than 90 %. Sequence distance is indicated by horizontal arm length, scale bar shown. The HAP1_N domains from the following sequences were used: human (H. sapiens HAP1: ENSP00000334002.4 aa108–428; TRAK1: ENSP00000328998.5 aa48–354; TRAK2: ENSP00000328875.3 48–353), Tasmanian devil (S. harrisii HAP1: XP_012403736.1 aa9–292), rat (R. norvegicus HAP1: ENSRNOP00000072494.1 aa81–403), dog (C. familiaris HAP1: ENSCAFP00000023416.4 aa100–465), opossum (M. domesticus HAP1: XP_007482271.1 aa9–292; TRAK1: ENSMODP00000013909.4 aa47–352; TRAK2: ENSMODP00000018999.3 aa48–353), zebrafish (D. rerio HAP1: ENSDARP00000099219.3 aa19–303; TRAK1: ENSDARP00000115803.1 aa1–250; TRAK2: ENSDARP00000133065.1 aa32–349), clawed frog (Xenopus tropicalis HAP1: ENSXETP00000027307.2 aa116–422; TRAK1: XP_012819699.1 aa36–296; TRAK2: ENSXETP00000061179.1 aa29–335), chicken (G. gallus HAP1: ENSGALP00000039222.2 aa122–425; TRAK1: ENSGALP00000019447.4 aa129–435; TRAK2: ENSGALP00000013618.4 aa30–333), anole lizard (A. carolinensis HAP1: XP_008111605.1 aa10–305; TRAK1: XP_008113765.1 aa33–254; TRAK2: ENSACAP00000001540.3 aa30–353), fruitfly (Drosophila melanogaster Milton: FBpp0297338.1 aa76–375)

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