amputation site 2 – anterior regeneration (observation only every 2
nd
day)
|
0
|
invagination of 1st remaining segment at wound site
|
2
|
blastema formation
|
4
|
prostomium with developing antenna, palps and eyes
|
6–16
|
two or three segments
|
18
|
sequential addition of further segments
|
amputations site 2 – posterior regeneration (observation only every 2
nd
day)
|
0 + 2
|
invagination of 1st remaining segment at wound site
|
4
|
pygidium with developing anal cirri and median papilla
|
6 onwards
|
sequential addition of segments
|
amputation site 3 – anterior regeneration
|
0–1
|
invagination of 1st remaining segment at wound site
|
2
|
blastema formation
|
3
|
blastema well-developed
|
4
|
prostomium with developing antenna, palps and eyes; two or three segments with tentacular cirri regenerating in 1st segment
|
6
|
pharynx present
|
7 onwards
|
growth of segments and structures
|
amputation site 3 – posterior regeneration
|
0–2
|
invagination of 1st remaining segment at wound site
|
3
|
blastema well-developed
|
4
|
pygidium with developing anal cirri and median papilla
|
5
|
addition of first new segment
|
6 onwards
|
addition of further new segments followed by their development
|