Fig. 6

a - ASTRAL species tree inference based on the Maximum Likelihood inferred individual gene trees from the 83 taxa Euctenizidae family/genus level dataset, comprising 581 loci and 334,436 bp. ASTRAL analyzes unrooted gene trees; tree was subsequently rooted at the branch leading to the non-Aphonopelma outgroup taxa. ASTRAL node support values = support based on the RAxML bootstrap support from all trees and all loci. b - Maximum Likelihood analysis of the Aphonopelma concatenated supermatrix. Black circles denote 100 % bootstrap support; black squares denote bootstrap support between 99-80 %; white squares denote bootstrap support less than 80 %. The species tree inference is likely the more appropriate evaluation of the data at this timescale. Inset key and colors denote taxonomic groupings recovered with our sampling. All genealogically exclusive species [79] are identified with a grey bar; A. iodius, a paraphyletic species as presently defined, is identified by the black boxes. Novel taxa are denoted by an asterisk. Lineages with a different placement between the two outcomes are indicated in yellow. Photographs illustrate the Aphonopelma species corresponding to that region of the phylogeny