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Fig. 5 | BMC Evolutionary Biology

Fig. 5

From: Evolution of long centromeres in fire ants

Fig. 5

Evolution of CenSol copy expansion in Solenopsis. a Slot blot hybridization to estimate the relative amounts of the CenSol sequence among seven genomes. The CenSol signals were normalized with the staining intensities of total DNA (500 ng) by methylene blue. The CenSol signals at unsaturated dilutions for each species (red boxes, 50 ng and 5 ng, rows 2 and 3) were first calculated relative to the shared S. daguerrei genome and then rescaled relative to the S. geminata genome. DNA amounts are labeled. The evolutionary relationships of the seven species are shown below. b PCR assay for detecting CenSol satellite in seven Solenopsis species. Genomic DNA concentrations used for PCR are indicated below. c Localization of CenSol by FISH on diploid interphase cells in five Solenopsis species. d A model for the evolution of CenSol copy number in Solenopsis. The CenSol levels for the fire ant species (numbers at tips; values from (a) were used to infer ancestral state levels for CenSol (numbers above nodes) using the linear-change parsimony model. Continuous CenSol values were manually split into four bins (colors), which placed the seven studied species into four groups based on their relative CenSol levels

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