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Fig. 2 | BMC Evolutionary Biology

Fig. 2

From: Evolution of long centromeres in fire ants

Fig. 2

a Localization of the first and the second most abundant satellites on the metaphase chromosomes of S. invicta and S. geminata. FISH analysis on haploid cells combining the CenSol probe (green) with the second most abundant satellite (Solmin, blue); chromosomes counterstained with DAPI (gray). b Summary of the length distributions of the CenSol repeat in the S. invicta and S. geminata draft genomes. BLASTN hits of the consensus CenSol sequence against the S. invicta and S. geminata genomes were binned by sequence length. The 109 bp unit was the dominant repeat length for both ants. c Sequence logos generated with 10,469 and 5423 unique 109 bp CenSol sequences from S. invicta and S. geminata, respectively. The height of each letter is proportional to the frequency of four nucleotides, adenine (A), thymine (T), guanine (G), and cytosine (C). The total height of each stack, measured in bits, is related to the binding energy [57]. EcoRV and BsaAI restriction sites are indicated with arrowheads. The BsaAI in parenthesis indicates a less common polymorphic cutting site in the CenSol monomers. The locations of the A1repV1 primers are shown

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